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Stage Ⅰ (before graduation): emphasis on fundamental researchMy research experience began with my PhD project, during which I studied theexpression and function of haloarchaeal hsp70 gene. The nucleotides flanking the startcodon in hsp70 mRNAs with very short 5'-UTRs were mutated, and theircorresponding influences on gene expression (including transcript, translation leveland translational efficiency) were analyzed. Moreover, three genes (grpE, hsp70 andhsp40) of the Hsp70 chaperone machine in Hfx. volcanii were cloned, deleted andwell characterized to analyze their function on the survival and growth of haloarchaeaunder the conditions of high temperature and low salinity.