刘夺
教师名称：刘夺
教师拼音名称：Liu Duo
出生日期：1987-04-01
性别：男
职称：讲师
其他联系方式
邮编：300072
通讯/办公地址：No. 92, Weijin Road, Nankai District, Tianjin
移动电话：13662113599
邮箱：liuduo19870401@126.com
基本信息
研究方向
获奖情况
论文成果
Liu Duo is a Ph.D majoring in synthetic biology. He has worked on the modular strategies of synthetic biology for combinatorial construction of yeast metabolic system for natural product biosynthesis. One of his main researches is focused on developing a new strategy (M-PERL) to introduce DNA sequence libraries into multigene pathway. This strategy utilizes small double-strand DNA as regulatory linkers to assist assembly of multiple gene parts. The regulatory linker contains a central regulatory region and two outside homologous ends, which were alternatively homologous with two orthogonal gene parts ready for assembly. With this strategy, several yeast promoter libraries covering varied strengths were developed. Alternative libraries inserting 5, 20, 40 random basepairs into five different promoters are used to regulate a five-gene pathway for violacein synthesis. Each gene expression and pathway’s product profiles are significantly regulated. This strategy is highly efficient for one-step assembly and regulation of multigene pathways. (Read article: DOI: 10.1021/acssynbio.6b00123)
modular engineering of metabolic pathways and metabolic system especially using heterologous gene combinations
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Liu D, Li BZ, Liu H, Guo XJ, Yuan YJ. Profiling influences of gene overexpression on heterologous resveratrol production in Saccharomyces cerevisiae. FRONT Chem Sci Eng, 11(1): 117-125(2017)..2017
Luo Y#, Li BZ#, Liu D#, Zhang L, Chen Y, Jia B, Zeng BX, Zhao H, Yuan YJ(# Equal contribution): Engineered biosynthesis of natural products in heterologous hosts. Chem Soc Rev, 44(15): 5265-5290(2015). .2017
Liu D, Liu H, Li BZ, Qi H, Jia B, Zhou X, Du HX, Zhang W, Yuan YJ: Multigene Pathway Engineering with Regulatory Linkers (M-PERL). ACS Synth Biol, 5(12): 1535-1545(2016)..2017
教育经历
2005.9-2009.7
Tianjin University Bioengineering Bachelor
2009.9-2017.1
Tianjin University Biochemical Engineering Ph.D
工作经历
2017.5
-2019.12
|Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology|Tianjin University |
